SearchLight is a free, online spectrum plotting and analysis tool that allows fluorescence microscope users and optical instrument designers to model & evaluate the spectral performance of fluorophores, filter sets, light sources, and detectors as components of an overall system. Its intuitive user interface makes it easy to manipulate spectral component specifications and plot spectral results. The System Calculator allows the user to quantify system metrics including signal, SNR, and bleed through; as well as to compare various system configurations against each other.

Any number of spectra may be plotted simultaneously, and an entire graphing session sent to colleagues for collaboration using the "Share" feature. SearchLight sessions can also be saved into your personal account for future use.

SearchLight contains an extensive library of 955 fluorescence spectrum models, 217 light sources, more than a dozen detectors, and Semrock's entire library of filters and filter sets. "MyData" allows you to upload your own custom datasets for any components not currently included as a selection choice.

• Evaluate and optimize your system configurations
• Save/Share/Export your results for better collaboration
• Take advantage of the regularly updated databases
• Save time in the lab and make every photon count with Searchlight!

For additional details visit SearchLight
For a video tutorial and helpful hints visit SearchLight spectrum plotting tool support

Start using SearchLight now!

Compare System Calculator

SearchLight Compare System Calculator feature quantitatively computes the fluorescence signal, noise, and signal-to-noise ratio for unlimited combinations of filter sets, fluorophores, light sources, and detectors. Calculations are based on a straightforward mathematical framework, which is described in detail in the white paper Spectral Modeling in Fluorescence Microscopy.